VETCARE ^ÔVETERINARY PRODUCTS

RAPID DIAGNOSTIC KITS

AQUAHEALTH ^ÔDIAGNOSTIC SERVICES

MONO-AQUA

RAPID-DIAGNOSTIC KITS FOR USE IN VITRO

Types:

• Mono-aqua -Va Vibriosis. Vibrio anguillarum
• Mono-aqua -Pp Pasteurellosis. Photobacterium damselae subsp. piscicida
• Mono-aqua -Yr Yersiniosis -ERM. Yersinia ruckeri

DESCRIPTION

The Mono-Aqua test kits comprise rapid agglutination tests, rapidly verifying (30 sec.) the presence of specific bacteria on cultures. Hence, they are destined for laboratory use requiring seeding samples on growth media. They are termed "in vitro" because they are not applied on live fish. The test reagent (specific antiserum) consists of mono-disperse particles coated with antibodies which form a visible granular particle agglutination pattern when mixed with the particular bacterium (antigen) that they are designed to diagnose.

Apart from the test reagent, the kits are delivered with instruction leaflet and analysis cards. In addition, they contain a negative control reagent, consisting also of mono-disperse particles coated with non-specific, but closely related proteins in order to verify the positive result obtained by the test reagent and exclude false positive results. Each kit suffices for 50 tests.

APPLICATION

The Mono-Aqua rapid agglutination tests are applicable for a rapid preliminary screening of the pathogens isolated on culture, hence speeding bacterial identification and the diagnosis of the most economically important diseases.

The tests are performed by mixing a drop of the test reagent with one or more bacterial colonies, removed from the agar plate, on the black background of an analysis card. A positive result (specific agglutination) is read as visible white granular particles (agglutinates) of equal size on a dark background, formed within 30sec. and homogeneously spread over the test zone on the analysis card. (Non-specific, spontaneous agglutination differs from ordinary agglutination by giving non homogeneous flakes as sticky agglutinates on a milky background. Such a result should be considered as negative.)

Verification of the positive result: In case of a positive result with the test reagent then the negative control reagent is similarly applied on a couple of bacterial colonies from the agar plate. This is to achieve maximum reliability since, occasionally, non-specific agglutination may occur. When a drop of the negative control reagent is mixed for 30sec with the bacterial colonies on the analysis card, it should not give an ordinary agglutination.

The Mono-Aqua rapid diagnostic kits are ideal for the fast and safe identification of specific bacteria as well as for the verification of their presence when grown on agar plates mixed with colonies of other bacterial strains.

RECOMMENDATIONS

• For laboratory use only. The test should not be performed under direct sun-light.
• Allow the reagents to reach room temperature (20^o-25^oC) before use.
• Shake the reagent vials vigorously before use. The reagent solutions must be homogeneous before use.
• Minimum sample quantity >1 bacterial colonies of about 1mm in diameter.
• Dispose off all specimens and materials used to perform the test by standard decontamination methods.

STORAGE

The Mono-aqua test kits should be stored refrigerated at +2^o to +7^o C. Do not freeze. They last in a common refrigerator for at least 36 months. They may be used several times according to needs. Always close vials properly after use.

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